Many short peptides selectively permeabilize the bacteria plasma membrane, leading to their lyses and death: they are therefore a source of antibacterial molecules and inspiration for novel and more selective drugs, which may have wider application in many other fields, as selective anticancer drugs. In this chapter, it is presented a new method to investigate the permeabilization properties of antimicrobial peptides under strict physiological conditions, employing the patch-clamp technique coupled to a fast perfusion system.

Studying the mechanism of membrane permeabilization induced by antimicrobial peptides using patch-clamp techniques

RISPOLI, Giorgio
2017

Abstract

Many short peptides selectively permeabilize the bacteria plasma membrane, leading to their lyses and death: they are therefore a source of antibacterial molecules and inspiration for novel and more selective drugs, which may have wider application in many other fields, as selective anticancer drugs. In this chapter, it is presented a new method to investigate the permeabilization properties of antimicrobial peptides under strict physiological conditions, employing the patch-clamp technique coupled to a fast perfusion system.
978-1-4939-6735-3
978-1-4939-6737-7
CHO-K1 cells; Ion channels; Photoreceptors; Pore-forming peptides; Pore-forming toxins; Rod outer segment; Whole-cell recording; Molecular Biology; Genetics
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11392/2363328
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