Background An increased ATP concentration is a typical biochemical feature of the tumor microenvironment (TME). Likewise, many malignant tumors overexpress plasma membrane receptors for extracellular ATP (P2Rs), and P2Rs play a role in cancer cell intravasation/extravasation and therefore in metastatic spreading. These observations highlight the importance of purinergic signalling in cancer research. A substantial breakthrough in the biochemistry and cell biology of extracellular ATP has been the generation of a bioluminescent probe allowing the in vivo real-time measurement of the extracellular ATP concentration. This probe, named plasma membrane luciferase (pmeLUC) has allowed to unequivocally demonstrate that the TME is a "high-ATP environment", and that ATP within this environment can be used by cancer cells to support their metabolic requirements. Among the P2Rs, P2X7R is mostly involved in cancer as: a) is upregulated in cancer cells; b) promotes growth; c) stimulates the Warburg effect; d) is necessary for metastatic spreading. In addition, P2X7R is intimately linked to the NLRP3 inflammasome.

The P2X7 receptor: a key determinant of cellular metabolic energetics in cancer progression - AIRC IG 18581

DI VIRGILIO, Francesco
2016

Abstract

Background An increased ATP concentration is a typical biochemical feature of the tumor microenvironment (TME). Likewise, many malignant tumors overexpress plasma membrane receptors for extracellular ATP (P2Rs), and P2Rs play a role in cancer cell intravasation/extravasation and therefore in metastatic spreading. These observations highlight the importance of purinergic signalling in cancer research. A substantial breakthrough in the biochemistry and cell biology of extracellular ATP has been the generation of a bioluminescent probe allowing the in vivo real-time measurement of the extracellular ATP concentration. This probe, named plasma membrane luciferase (pmeLUC) has allowed to unequivocally demonstrate that the TME is a "high-ATP environment", and that ATP within this environment can be used by cancer cells to support their metabolic requirements. Among the P2Rs, P2X7R is mostly involved in cancer as: a) is upregulated in cancer cells; b) promotes growth; c) stimulates the Warburg effect; d) is necessary for metastatic spreading. In addition, P2X7R is intimately linked to the NLRP3 inflammasome.
2016
Nazionale
Responsabile di Unità locale
AIRC
DI VIRGILIO, Francesco
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11392/2363129
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact