Crocin, a potent antioxidant obtained fromsaffron, shows anticancer activity in in vivo models. Unfortunately unfavorable physicochemical features compromise its use in topical therapy. The present study describes the preparation and characterization of nanostructured lipid dispersions as drug delivery systems for topical administration of crocin and the evaluation of antioxidant and anti proliferative effects of crocin once encapsulated into nanostructured lipid dispersions. Nanostructured lipid dispersions based onmonoolein in mixturewith sodiumcholate and sodiumcaseinate have been characterized by cryo-TEM and PCS. Crocin permeation was evaluated in vitro by Franz cells, while the oxygen radical absorbance capacity assay was used to evaluate the antioxidant activity. Furthermore, the anti proliferative activity was tested in vitro by the MTT test using a human melanoma cell line. The emulsification of monoolein with sodium cholate and sodium caseinate led to dispersions of cubosomes, hexasomes, sponge systems and vesicles, depending on the employed emulsifiers. Permeation and shelf life studies demonstrated that nanostructured lipid dispersions enabled to control both rate of crocin diffusion through the skin and crocin degradation. The oxygen radical absorbance capacity assay pointed out an interesting and prolonged antioxidant activity of crocinwhile the MTT test showed an increase of crocin cytotoxic effect after incorporation in nanostructured lipid dispersions. Thiswork has highlighted that nanostructured lipid dispersions can protect the labilemolecule crocin from degradation, control its skin diffusion and prolong antioxidant activity, therefore suggesting the suitability of nano structured lipid dispersions for crocin topical administration.
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|Titolo:||Nanostructured lipid dispersions for topical administration of crocin, a potent antioxidant from saffron (Crocus sativus L.)|
|Data di pubblicazione:||2017|
|Appare nelle tipologie:||03.1 Articolo su rivista|