Chemicals discovered in water at levels that may be significantly different than expected are referred to as contaminants of emerging concern (CECs) because the risk to environmental health posed by their occurrence/frequency is still unknown. The worldwide distributed compounds perfluorooctanoic acid (PFOA) and bisphenol A (BPA) may fall into this category due to effects on endocrine receptors. We applied an ex vivo assay using small slices of bioptic skin from the bottlenose dolphin, Tursiops truncatus, cultured and treated for 24 h with different PFOA or BPA concentrations to analyze global gene expression. RNA was labeled and hybridized to a species-specific oligomicroarray. The skin transcriptome held information on the contaminant exposure, potentially predictive about long-term effects on health, being the genes affected involved in immunity modulation, response to stress, lipid homeostasis, and development. The transcriptomic signature of dolphin skin could be therefore relevant as classifier for a specific contaminant.
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|Titolo:||Transcriptomic analysis of dolphin (Tursiops truncatus) skin biopsies: a new approach to understand the effects of emerging contaminants on apex predators.|
|Data di pubblicazione:||2016|
|Appare nelle tipologie:||03.1 Articolo su rivista|