Structural fat grafting utilizes the centrifugation of liposuction aspirates to create a graded density of adipose tissue. This study was performed to qualitatively investigate the effects of centrifugation on stem cells present in adipose tissue. Liposuction aspirates were obtained from healthy donors and either not centrifuged or centrifuged at 1,800 rpm for 3 minutes. The obtained fat volumes were divided into three layers and then analyzed. The results demonstrate that centrifugation induces a different distribution of stem cells in the three layers. The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers. The low-density layer exhibits an enrichment of multipotent stem cells. We conclude that appropriate centrifugation concentrates stem cells. This finding may influence the clinical practice of liposuction aspirate centrifugation and enhance graft uptake. © 2014 Clauser et al.

Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery

CLAUSER, Luigi
Co-primo
;
TIEGHI, Riccardo;Galiè, Manlio;ELIA, Giovanni;PINTON, Paolo;ZAVAN, Barbara
Ultimo
2014

Abstract

Structural fat grafting utilizes the centrifugation of liposuction aspirates to create a graded density of adipose tissue. This study was performed to qualitatively investigate the effects of centrifugation on stem cells present in adipose tissue. Liposuction aspirates were obtained from healthy donors and either not centrifuged or centrifuged at 1,800 rpm for 3 minutes. The obtained fat volumes were divided into three layers and then analyzed. The results demonstrate that centrifugation induces a different distribution of stem cells in the three layers. The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers. The low-density layer exhibits an enrichment of multipotent stem cells. We conclude that appropriate centrifugation concentrates stem cells. This finding may influence the clinical practice of liposuction aspirate centrifugation and enhance graft uptake. © 2014 Clauser et al.
2014
Clauser, Luigi; Ferroni, Letizia; Gardin, Chiara; Tieghi, Riccardo; Galiè, Manlio; Elia, Giovanni; Piattelli, Adriano; Pinton, Paolo; Bressan, Eribert...espandi
File in questo prodotto:
File Dimensione Formato  
160.pdf

accesso aperto

Descrizione: versione editoriale
Licenza: Creative commons
Dimensione 3.54 MB
Formato Adobe PDF
3.54 MB Adobe PDF Visualizza/Apri

I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2334609
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 14
social impact