In this paper, the electric field generated in a conductive medium by needles pairs with inter-needle distance of 1 or 2 cm has been studied by means of 3D numerical models. The computational results have been correlated with experimental ones obtained in vitro on cultured cells. In particular, electroporation effect has been assessed on human osteosarcoma cell line MG63 cultured in monolayer. These results are helpful to evaluate the effect of the needle distance on the electric field distribution in cultured cells in terms of occurrence of reversible or irreversible electroporation.

Effect of electrode distance in electrochemotherapy: From numerical model to in vitro tests

ONGARO, Alessia
Primo
;
DE MATTEI, Monica;PELLATI, Agnese;
2016

Abstract

In this paper, the electric field generated in a conductive medium by needles pairs with inter-needle distance of 1 or 2 cm has been studied by means of 3D numerical models. The computational results have been correlated with experimental ones obtained in vitro on cultured cells. In particular, electroporation effect has been assessed on human osteosarcoma cell line MG63 cultured in monolayer. These results are helpful to evaluate the effect of the needle distance on the electric field distribution in cultured cells in terms of occurrence of reversible or irreversible electroporation.
2016
978-981-287-816-8
978-981-287-817-5
978-981-287-816-8
978-981-287-817-5
Electroporation, electric field, electrode, propidium iodide, reversible and irreversible electroporation
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2330152
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