Objectives - We studied the in vitro production of variably MS-related cytokines from Sardinian MS and healthy donors bearing the two 'Sardinian' MS-associated HLA-DR alleles: DR3 and DR4, with the purpose to evidentiate possible differences in their immune response. Materials and methods - ELISA were used for detection of cellular products by mitogen-activated peripheral blood mononuclear cells. Results - PHA-activated HLA-DR4+/DR3- mononuclear cells produce significantly higher amounts of TNF-α compared with the DR3+/DR4-. In addition, homozygous HLA-DR3 + mononuclear cells from MS patients produce significantly lower amounts of IL-10 than those from homozygous HLA-DR3 + healthy donors. Conclusion - The abnormal production of detrimental or regulatory cytokines may account for the genetic susceptibility to MS in different HLA-subgroups of Sardinian MS patients.
Genetic susceptibility to multiple sclerosis in Sardinians: an immunological study
PUGLIATTI, Maura;
1998
Abstract
Objectives - We studied the in vitro production of variably MS-related cytokines from Sardinian MS and healthy donors bearing the two 'Sardinian' MS-associated HLA-DR alleles: DR3 and DR4, with the purpose to evidentiate possible differences in their immune response. Materials and methods - ELISA were used for detection of cellular products by mitogen-activated peripheral blood mononuclear cells. Results - PHA-activated HLA-DR4+/DR3- mononuclear cells produce significantly higher amounts of TNF-α compared with the DR3+/DR4-. In addition, homozygous HLA-DR3 + mononuclear cells from MS patients produce significantly lower amounts of IL-10 than those from homozygous HLA-DR3 + healthy donors. Conclusion - The abnormal production of detrimental or regulatory cytokines may account for the genetic susceptibility to MS in different HLA-subgroups of Sardinian MS patients.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
