We investigated the effect of high-frequency electromagnetic fields (HF-EMF) and 17-β-estradiol on connexins(Cxs), integrins(Ints) and estrogen receptor (ER) expression as well as on ultrastructure of trophoblast-derived HTR-8/SVneocells. HF-EMF, 17-β-estradioland their combination induced an increase of Cx40 and Cx43 mRNA expression. HF-EMF decreased Intalpha and β mRNA levels, but enhanced IntalphamRNA expression. All the IntsmRNA expressions were increased by 17-β-estradiol and exposure to both stimuli. ER-mRNA was reduced by HF-EMF, but augmented by 17-β-estradiolalone or with HF-EMF. ER-immuno-fluorescence showed a cytoplasmic localization in sham and HF-EMF exposed cells which became nuclear after treatment with hormone or both stimuli. Electron microscopy evidenced a loss of cellular contact in exposed cells which appeared counteracted by 17-β-estradiol. Wedemonstrate that 17-β-estradiol modulatesCxs and Intsas well asER-expressioninduced by HF- EMF, suggesting an influence of both stimuli on trophoblast differentiation and migration.

17- β -estradiol counteracts the effects of high frequency electromagnetic fields on trophoblastic connexins and integrins

CERVELLATI, Franco;VALACCHI, Giuseppe;LUNGHI, Laura;MARCI, Roberto;BIONDI, Carla;VESCE, Fortunato
2013

Abstract

We investigated the effect of high-frequency electromagnetic fields (HF-EMF) and 17-β-estradiol on connexins(Cxs), integrins(Ints) and estrogen receptor (ER) expression as well as on ultrastructure of trophoblast-derived HTR-8/SVneocells. HF-EMF, 17-β-estradioland their combination induced an increase of Cx40 and Cx43 mRNA expression. HF-EMF decreased Intalpha and β mRNA levels, but enhanced IntalphamRNA expression. All the IntsmRNA expressions were increased by 17-β-estradiol and exposure to both stimuli. ER-mRNA was reduced by HF-EMF, but augmented by 17-β-estradiolalone or with HF-EMF. ER-immuno-fluorescence showed a cytoplasmic localization in sham and HF-EMF exposed cells which became nuclear after treatment with hormone or both stimuli. Electron microscopy evidenced a loss of cellular contact in exposed cells which appeared counteracted by 17-β-estradiol. Wedemonstrate that 17-β-estradiol modulatesCxs and Intsas well asER-expressioninduced by HF- EMF, suggesting an influence of both stimuli on trophoblast differentiation and migration.
Cervellati, Franco; Valacchi, Giuseppe; Lunghi, Laura; Fabbri, E.; Valbonesi, P.; Marci, Roberto; Biondi, Carla; Vesce, Fortunato
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1814508
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