Inactivation of 6PGDH by glyoxilate requires the presence of a carboxyl group, an aldehydic group and a specific reducing agent, cyanoborohydride. It appears that the inactivation is due to an affinity labelling, but a lysine nearby is required. Other enzymes binding anionic ligands are inactivated but two enzymes, which use glyoxilate as a substrate, are not inactivated by glyoxilate and cyanoborohydride. Glyoxilate is thus useful to identify specific lysines at the carboxyl binding sites in proteins.
Glyoxylate for affinity labelling of 6-phosphogluconate dehydrogenase
HANAU, Stefania;BERTELLI, Massimo
1994
Abstract
Inactivation of 6PGDH by glyoxilate requires the presence of a carboxyl group, an aldehydic group and a specific reducing agent, cyanoborohydride. It appears that the inactivation is due to an affinity labelling, but a lysine nearby is required. Other enzymes binding anionic ligands are inactivated but two enzymes, which use glyoxilate as a substrate, are not inactivated by glyoxilate and cyanoborohydride. Glyoxilate is thus useful to identify specific lysines at the carboxyl binding sites in proteins.File in questo prodotto:
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