The efficacy of different therapies and vaccine preparations was assessed for treating or preventing herpetic ocular keratitis induced by experimental inoculation in rabbits with two HSV-1 variants that display different pathogenetic potential. Early administration of acyclovir (ACV) promoted fast healing and prevented neurologic involvements: alpha-interferon (alpha-IFN) was less efficient than ACV; combined therapy with both drugs increased the antiviral effects. In an attempt to prevent the disease, rabbits were vaccinated with a slightly pathogenic HSV-1 variant or with a secreted form of an engineered HSV-1 glycoprotein gB (gB-1s) and were subsequently challenged with a highly pathogenic HSV-1 variant. Immunization of rabbits with gB-1s was much more efficient than immunization with live virus in reducing the severity of herpetic keratitis and in preventing CNS disease.

Experimental keratitis in rabbits by human HSV-1 variants: prevention andtreatment

MANSERVIGI, Roberto;INCORVAIA, Carlo;DI LUCA, Dario;ROTOLA, Antonella;BALBONI, Pier Giorgio;SEBASTIANI, Adolfo;ROSSI, Antonio;CASSAI, Enzo
1990

Abstract

The efficacy of different therapies and vaccine preparations was assessed for treating or preventing herpetic ocular keratitis induced by experimental inoculation in rabbits with two HSV-1 variants that display different pathogenetic potential. Early administration of acyclovir (ACV) promoted fast healing and prevented neurologic involvements: alpha-interferon (alpha-IFN) was less efficient than ACV; combined therapy with both drugs increased the antiviral effects. In an attempt to prevent the disease, rabbits were vaccinated with a slightly pathogenic HSV-1 variant or with a secreted form of an engineered HSV-1 glycoprotein gB (gB-1s) and were subsequently challenged with a highly pathogenic HSV-1 variant. Immunization of rabbits with gB-1s was much more efficient than immunization with live virus in reducing the severity of herpetic keratitis and in preventing CNS disease.
Manservigi, Roberto; Incorvaia, Carlo; DI LUCA, Dario; Rotola, Antonella; Balboni, Pier Giorgio; Sebastiani, Adolfo; Rossi, Antonio; Cassai, Enzo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11392/1722162
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