Regulation of gene expression is operated, at transcriptional level, by the interactions between proteins (transcription factors) and elements present within eukaryotic and viral promoters (transcription signals) exhibiting specific nucleotide sequences. In this study we performed DNase I cleavage and analysis of the cleavage products using the Pharmacia ALF(TM) DNA sequencing system. As model system we employed the long terminal repeat (LTR) of the human immunodeficiency type I (HIV-1) virus, containing the DNA sequences recognized by a number of transcription factors, including NF-kB, Sp1 and TFIID. The main conclusion of our experiments is that automated analysis of DNAse I footprinting employing the ALF DNA sequencing system is a fast and reliable technique to study protein-DNA interactions

A non-radioactive automated protocol to study protein-DNA interactions by DNase I footprinting

MISCHIATI, Carlo;FERIOTTO, Giordana;BIANCHI, Nicoletta;GAMBARI, Roberto
1995

Abstract

Regulation of gene expression is operated, at transcriptional level, by the interactions between proteins (transcription factors) and elements present within eukaryotic and viral promoters (transcription signals) exhibiting specific nucleotide sequences. In this study we performed DNase I cleavage and analysis of the cleavage products using the Pharmacia ALF(TM) DNA sequencing system. As model system we employed the long terminal repeat (LTR) of the human immunodeficiency type I (HIV-1) virus, containing the DNA sequences recognized by a number of transcription factors, including NF-kB, Sp1 and TFIID. The main conclusion of our experiments is that automated analysis of DNAse I footprinting employing the ALF DNA sequencing system is a fast and reliable technique to study protein-DNA interactions
Mischiati, Carlo; Feriotto, Giordana; Bianchi, Nicoletta; Gambari, Roberto
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1690798
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? ND
social impact