The selectivity properties of methanol, acetonitrile and tetrahydrofuran as organic modifiers in the reversed-phase high-performance liquid chromatographic separation of flavonoid compounds was studied. Conditions for achieving separations between compound classes are described, in particular the possible separation of glycosides from aglycones in acetonitrile and tetrahydrofuran. Eleven retention contributions as Δlog k′ are reported, and their dependence on mobile-phase composition is described. Solvent strength values and useful gradient elution conditions are given.
Solvent Selectivity Effects In Reversed-phase High-performance Liquid-chromatography of Flavonoid Compounds
DONDI, Francesco
Primo
;G. BLO;PIETROGRANDE, Maria Chiara;
1989
Abstract
The selectivity properties of methanol, acetonitrile and tetrahydrofuran as organic modifiers in the reversed-phase high-performance liquid chromatographic separation of flavonoid compounds was studied. Conditions for achieving separations between compound classes are described, in particular the possible separation of glycosides from aglycones in acetonitrile and tetrahydrofuran. Eleven retention contributions as Δlog k′ are reported, and their dependence on mobile-phase composition is described. Solvent strength values and useful gradient elution conditions are given.File in questo prodotto:
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