Adult mesenchymal stem cells derived from adipose tissue (A-MSC) have the capacity to differentiate in vitro into mesenchymal as well as endodermal and ectodermal cell lineages. We investigated the neural differentiation potential of human A-MSC with a protocol which included sphere formation and sequential culture in brain-derived neurotrophic factor (BDNF) and retinoic acid (RA). After 30 days, 57% A-MSC showed morphological, immunocytochemical and electrophysiological evidence of initial neuronal differentiation. In fact, A-MSC had spherical shape with protrusion of two or three cellular processes; by immunocytochemistry, these elements expressed nestin and neuronal molecules (MAP-2 and NeuN) in the absence of glial phenotypic markers. Differentiated cells showed negative membrane potential (-70 mV), delayed rectifier potassium currents and TTX-sensitive sodium currents. Such changes were stable for at least 7 days after removal of differentiation medium. In view of this results and the easy availability of adipose tissue, A-MSC may be an useful and ready source of adult mesenchymal stem cells (MSC).
Neuronal differentiation potential of human adipose-derived mesenchymal stem cells
PIGNATELLI, Angela;BELLUZZI, Ottorino;
2007
Abstract
Adult mesenchymal stem cells derived from adipose tissue (A-MSC) have the capacity to differentiate in vitro into mesenchymal as well as endodermal and ectodermal cell lineages. We investigated the neural differentiation potential of human A-MSC with a protocol which included sphere formation and sequential culture in brain-derived neurotrophic factor (BDNF) and retinoic acid (RA). After 30 days, 57% A-MSC showed morphological, immunocytochemical and electrophysiological evidence of initial neuronal differentiation. In fact, A-MSC had spherical shape with protrusion of two or three cellular processes; by immunocytochemistry, these elements expressed nestin and neuronal molecules (MAP-2 and NeuN) in the absence of glial phenotypic markers. Differentiated cells showed negative membrane potential (-70 mV), delayed rectifier potassium currents and TTX-sensitive sodium currents. Such changes were stable for at least 7 days after removal of differentiation medium. In view of this results and the easy availability of adipose tissue, A-MSC may be an useful and ready source of adult mesenchymal stem cells (MSC).I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
