We recently published a highly reproducible protocol that permitted to isolate, culture and expand human Multipotent Adult Stem Cells (hMASCs) from human liver, heart and bone marrow (Beltrami et al., 2007). Although their high proliferative rate and differentiation ability make MASC dependable candidates for regenerative medicine applications, they do not represent a convenient source of cells for the inherent limits dictated by their scarce accessibility. Looking for more convenient alternatives, we applied the same isolation protocol to obtain MASCs from adipose tissue. Adipose Tissuederived MASCs (AT‐MASCs) can represent a valid substitute to Heart‐derived MASCs (H‐MASCs) because readily accessible, highly proliferative and widely multipotent, being able to differentiate into mesenchymal and non‐ mesenchymal lineages. In addition, the number of cells obtained by lipoaspirates is usually largely sufficient for many clinical uses. With the aim to explore possible therapeutic applications of MASCs in cardiac regenerative medicine, we compared ATMASCs and H‐MASCs for their in vitro myogenic differentiation potential. Several induction protocols were applied, and in order to compare their efficiency we evaluated the degree of myogenic differentiation under different morphological, functional and electrophysiological aspects.
Morphological and functional analysis of cardiomyocytes differentiated from adipose-derived human adult mesenchymal stem cells
PIGNATELLI, Angela;GAMBARDELLA, Cristina;BELLUZZI, Ottorino
2008
Abstract
We recently published a highly reproducible protocol that permitted to isolate, culture and expand human Multipotent Adult Stem Cells (hMASCs) from human liver, heart and bone marrow (Beltrami et al., 2007). Although their high proliferative rate and differentiation ability make MASC dependable candidates for regenerative medicine applications, they do not represent a convenient source of cells for the inherent limits dictated by their scarce accessibility. Looking for more convenient alternatives, we applied the same isolation protocol to obtain MASCs from adipose tissue. Adipose Tissuederived MASCs (AT‐MASCs) can represent a valid substitute to Heart‐derived MASCs (H‐MASCs) because readily accessible, highly proliferative and widely multipotent, being able to differentiate into mesenchymal and non‐ mesenchymal lineages. In addition, the number of cells obtained by lipoaspirates is usually largely sufficient for many clinical uses. With the aim to explore possible therapeutic applications of MASCs in cardiac regenerative medicine, we compared ATMASCs and H‐MASCs for their in vitro myogenic differentiation potential. Several induction protocols were applied, and in order to compare their efficiency we evaluated the degree of myogenic differentiation under different morphological, functional and electrophysiological aspects.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
