The reaction between 6-phosphogluconate dehydrogenase from Candida Utilis and 5,5' dithiobis(2-nitrobenzoate) results in the inactivation of the enzyme. At pH 6.0 the inactivation can be correlated with the modification of only one SH group per enzyme subunit. The modified SH group can react with another SH group forming and intramolecular disulfide bridge. The results obtained suggest that the SH group of two cysteine residues are located close in the tridimensional structure of the enzyme

Evidences for the proximity of two sulfhydryl groups in the active site of 6-phosphogluconate dehydrogenase

RIPPA, Mario;SIGNORINI, Marco;DALLOCCHIO, Franco Pasquale Filippo
1978

Abstract

The reaction between 6-phosphogluconate dehydrogenase from Candida Utilis and 5,5' dithiobis(2-nitrobenzoate) results in the inactivation of the enzyme. At pH 6.0 the inactivation can be correlated with the modification of only one SH group per enzyme subunit. The modified SH group can react with another SH group forming and intramolecular disulfide bridge. The results obtained suggest that the SH group of two cysteine residues are located close in the tridimensional structure of the enzyme
1978
Rippa, Mario; Signorini, Marco; Pernici, A.; Dallocchio, Franco Pasquale Filippo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1683830
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