It has been recently reported that, treating TPN with periodate, there is the oxidation to aldehydic groups of the two alcoholic groups present at carbons 2’ and 3’ of the ribose ring bound to the nicotinamide; this periodate oxidized TPN can be bound covalently to a resin to obtain a resin derivative useful for affinity chromatography of dehydrogenases [2] . We have now found that periodate oxidized TPN can be used efficiently as a powerful inhibitor specific for the TPN binding site of 6-phosphogluconate dehydrogenase from Candida utilis.
A NEW POWERFUL INHIBITOR SPECIFIC FOR THE TPN BINDING SITE OF 6-PHOSPHOGLUCONATE DEHY DROGENASE
RIPPA, Mario;SIGNORINI, Marco;DALLOCCHIO, Franco Pasquale Filippo
1975
Abstract
It has been recently reported that, treating TPN with periodate, there is the oxidation to aldehydic groups of the two alcoholic groups present at carbons 2’ and 3’ of the ribose ring bound to the nicotinamide; this periodate oxidized TPN can be bound covalently to a resin to obtain a resin derivative useful for affinity chromatography of dehydrogenases [2] . We have now found that periodate oxidized TPN can be used efficiently as a powerful inhibitor specific for the TPN binding site of 6-phosphogluconate dehydrogenase from Candida utilis.File in questo prodotto:
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