In the tritium exchange reaction catalysed by 6-phosphogluconate dehydrogenase from Candida utilis, the natural reduced coenzyme, 1-4 TPNH, has been substituted efficiently by its non enzymatically oxidizable isomer, 1-4 TPNH. This indicates that, in the tritium exchange reaction, the TPNH has not a redox but a structural role. We suggest that, as for 6-phosphogluconate dehydrogenase, isomers of the natural coenzymes may be used to differentiate, and study separately, the redox and structural roles of pyridine coenzymes in dehydrogenases. © 1972.
In the tritium exchange reaction catalysed by 6-phosphogluconate dehydrogenase from Candida utilis, the natural reduced coenzyme, 1-4 TPNH, has been substituted efficiently by its non enzymatically oxidizable isomer, 1-4 TPNH. This indicates that, in the tritium exchange reaction, the TPNH has not a redox but a structural role. We suggest that, as for 6-phosphogluconate dehydrogenase, isomers of the natural coenzymes may be used to differentiate, and study separately, the redox and structural roles of pyridine coenzymes in dehydrogenases. © 1972.
Differentiation between the structural and redox roles of TPNH in 6-phosphogluconate dehydrogenase
RIPPA, Mario;SIGNORINI, Marco;DALLOCCHIO, Franco Pasquale Filippo
1972
Abstract
In the tritium exchange reaction catalysed by 6-phosphogluconate dehydrogenase from Candida utilis, the natural reduced coenzyme, 1-4 TPNH, has been substituted efficiently by its non enzymatically oxidizable isomer, 1-4 TPNH. This indicates that, in the tritium exchange reaction, the TPNH has not a redox but a structural role. We suggest that, as for 6-phosphogluconate dehydrogenase, isomers of the natural coenzymes may be used to differentiate, and study separately, the redox and structural roles of pyridine coenzymes in dehydrogenases. © 1972.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
