The potential use of polymerase chain reaction (PCR)-generated DNA fragments (PCR-DNAs) as pharmaceutical agents has previously been suggested, with the demonstration of the in vitro cellular internalization and biologic activity of PCR-DNA decoy molecules targeted to human estrogen receptor gene. In order to provide information on the stability of these double-stranded DNA molecules, the nuclease resistance of PCR-DNAs of different sizes was studied in different conditions and experiments.

In vitro stability of polymerase chain reaction-generated DNA fragments in serum and cell extracts

PIVA, Maria Roberta
Primo
;
LAMBERTINI, Elisabetta
Secondo
;
PENOLAZZI, Maria Letizia;AGUIARI, Gianluca;NASTRUZZI, Claudio
Penultimo
;
DEL SENNO, Laura
Ultimo
1998

Abstract

The potential use of polymerase chain reaction (PCR)-generated DNA fragments (PCR-DNAs) as pharmaceutical agents has previously been suggested, with the demonstration of the in vitro cellular internalization and biologic activity of PCR-DNA decoy molecules targeted to human estrogen receptor gene. In order to provide information on the stability of these double-stranded DNA molecules, the nuclease resistance of PCR-DNAs of different sizes was studied in different conditions and experiments.
1998
Piva, Maria Roberta; Lambertini, Elisabetta; Penolazzi, Maria Letizia; M. C., Facciolo; A., Lodi; Aguiari, Gianluca; Nastruzzi, Claudio; DEL SENNO, Laura
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1683148
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