Aniline blue (AB) fluorochrome, a dye having a strong affinity for 1,3-beta-glucans, was assayed on growing cells of Schizosaccharomyces pombe, a fission yeast in which these polysaccharides constitute the main structural wall components. After 12 h exposure to 0.5 mg/ml of the dye, the yeasts showed some morphogenetic malformations which could be detected under UV and by transmission electron microscope. The most frequent aspects observed concerned the presence of multiseptate organisms in which non-cleavable septa were often thicker than usual, abnormally shaped and intensely fluorescent. The results suggest that AB interacts strongly with septum 1,3-beta-glucans, without preventing their synthesis and septum development. The block of the cleavage is tentatively explained by assuming that the beta-glucan-fluorochrome complexes are not recognized by the beta-glucanases whose activity is necessary for the partial hydrolysis of the polymers prior to cell separation.
Morphological changes induced by aniline blue in Schizosaccharomyces pombe
PANCALDI, Simonetta;DALL'OLIO, Giuseppe;POLI, Ferruccio;FASULO, Maria Palmira;VANNINI, Gian Luigi
1989
Abstract
Aniline blue (AB) fluorochrome, a dye having a strong affinity for 1,3-beta-glucans, was assayed on growing cells of Schizosaccharomyces pombe, a fission yeast in which these polysaccharides constitute the main structural wall components. After 12 h exposure to 0.5 mg/ml of the dye, the yeasts showed some morphogenetic malformations which could be detected under UV and by transmission electron microscope. The most frequent aspects observed concerned the presence of multiseptate organisms in which non-cleavable septa were often thicker than usual, abnormally shaped and intensely fluorescent. The results suggest that AB interacts strongly with septum 1,3-beta-glucans, without preventing their synthesis and septum development. The block of the cleavage is tentatively explained by assuming that the beta-glucan-fluorochrome complexes are not recognized by the beta-glucanases whose activity is necessary for the partial hydrolysis of the polymers prior to cell separation.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
