A rapid procedure for purification of myelin basic protein has been developed. White matter is delipidated with 2-butanol, and the residue is extracted at pH 7.5 and 8.5. Myelin basic protein is solubilized by extraction in acetate buffer, pH 4.5. The entire procedure requires less than 4 h, and gives homogeneous protein essentially free of protease activity. This procedure can be scaled down to process milligram amounts of white matter; thus it can be very useful for purification of myelin basic protein from very limited amounts of human white matter obtained during surgery.

A rapid method for purification of myelin basic protein

BELLINI, Tiziana;RIPPA, Mario;DALLOCCHIO, Franco Pasquale Filippo
1986

Abstract

A rapid procedure for purification of myelin basic protein has been developed. White matter is delipidated with 2-butanol, and the residue is extracted at pH 7.5 and 8.5. Myelin basic protein is solubilized by extraction in acetate buffer, pH 4.5. The entire procedure requires less than 4 h, and gives homogeneous protein essentially free of protease activity. This procedure can be scaled down to process milligram amounts of white matter; thus it can be very useful for purification of myelin basic protein from very limited amounts of human white matter obtained during surgery.
Bellini, Tiziana; Rippa, Mario; Matteuzzi, M.; Dallocchio, Franco Pasquale Filippo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1681372
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