The effect of the DNA demethylating agent 5-azacytidine (5-azaC) on the morphological development of Candida albicans blastospores has been investigated by microscopic observations. It was found that this compound does not produce any morphogenetic effect when the cells are not induced to mycelial form. By contrast, on the induced cells, 5-azaC markedly accelerates the process of germ tube formation. In addition in the treated cells, yeast-mycelium conversion develops synchronously, whereas it is asynchronous and slow in the normal cells. These data indicate that, together with phenotypic modifications, modulation of gene activity by DNA demethylation occurs in Candida albicans during morphogenetic changes.

5-azacytidine accelerates yeast-mycelium conversion in Candida albicans

PANCALDI, Simonetta;DEL SENNO, Laura;FASULO, Maria Palmira;POLI, Ferruccio;VANNINI, Gian Luigi
1988

Abstract

The effect of the DNA demethylating agent 5-azacytidine (5-azaC) on the morphological development of Candida albicans blastospores has been investigated by microscopic observations. It was found that this compound does not produce any morphogenetic effect when the cells are not induced to mycelial form. By contrast, on the induced cells, 5-azaC markedly accelerates the process of germ tube formation. In addition in the treated cells, yeast-mycelium conversion develops synchronously, whereas it is asynchronous and slow in the normal cells. These data indicate that, together with phenotypic modifications, modulation of gene activity by DNA demethylation occurs in Candida albicans during morphogenetic changes.
Pancaldi, Simonetta; DEL SENNO, Laura; Fasulo, Maria Palmira; Poli, Ferruccio; Vannini, Gian Luigi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1681166
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