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EnglishA three-dimensional network of structural filaments was visible with common electron microscopes in the cytoplasm of Euglena gracilis green cells extracted with buffers containing the nonionic detergent Triton X-100. A similar filamentous web was detected at the periphery of critical point dried cells cleaved on grids by means of an adhesive tape. SDS-polyacrylamide gel electrophoresis of the detergent-resistent cytoskeleton showed that actin or actin-like proteins of molecular weight in the range of 43–45 K are not among the components having a structural role inEuglena. The significance of these findings was discussed in relation to the capability of the alga to change the cell shape.
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| Titolo: | Cytoskeletal structures in Euglena gracilis after Triton X-100 extraction and dry cleaving | |
| Autori: | ||
| Data di pubblicazione: | 1985 | |
| Rivista: | ||
| Abstract: | A three-dimensional network of structural filaments was visible with common electron microscopes in the cytoplasm of Euglena gracilis green cells extracted with buffers containing the nonionic detergent Triton X-100. A similar filamentous web was detected at the periphery of critical point dried cells cleaved on grids by means of an adhesive tape. SDS-polyacrylamide gel electrophoresis of the detergent-resistent cytoskeleton showed that actin or actin-like proteins of molecular weight in the range of 43–45 K are not among the components having a structural role inEuglena. The significance of these findings was discussed in relation to the capability of the alga to change the cell shape. | |
| Handle: | http://hdl.handle.net/11392/1681163 | |
| Appare nelle tipologie: | 03.1 Articolo su rivista |
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