Peptide-15 (P-15) is an analog of the cell binding domain of collagen, has been shown to facilitate physiological process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. In this study we investigated the osteo-inductives properties of P15 on stem cells derived from bone marrow. In Real Time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR) we quantified the mRNA expression of specific genes, like transcriptional factors (RUNX2 and SP7), bone related genes (SPP1, COL1A1, COL3A1, ALPL, and FOSL1) and mesenchymal stem cells marker (ENG). Gene expression in BMSC was then compared with the gene expression in Human Osteoblasts (HOb) treated with P15, to evaluate the potential effect of this biomaterial in osteoblasts differentiation.
P15 INDUCES RUNX2 IN BONE MARROW DERIVED STEM CELLS
D. LAURITANO;CARINCI, Francesco;ZOLLINO, Ilaria;PALMIERI, Annalisa;ZAMBONI, Paolo;
2012
Abstract
Peptide-15 (P-15) is an analog of the cell binding domain of collagen, has been shown to facilitate physiological process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. In this study we investigated the osteo-inductives properties of P15 on stem cells derived from bone marrow. In Real Time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR) we quantified the mRNA expression of specific genes, like transcriptional factors (RUNX2 and SP7), bone related genes (SPP1, COL1A1, COL3A1, ALPL, and FOSL1) and mesenchymal stem cells marker (ENG). Gene expression in BMSC was then compared with the gene expression in Human Osteoblasts (HOb) treated with P15, to evaluate the potential effect of this biomaterial in osteoblasts differentiation.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
