Synthetic and biological materials are increasingly used to provide temporary or permanent scaffolds for bone regeneration. Allogro, a demineralized freeze-dried bone allograft is an optimal scaffold used worldwide to fill bone defects and to restore bone loss in orthopedic and maxillofacial surgery. To study how Allogro can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction. The obtained results demonstrated that Allogro influences the osteo-differentiation of stem cells as demonstrated by the activation of osteoblast related genes RUNX2, ALPL and SPP1.
ALLOGRO INDUCES OSTEOBLAST DIFFERENTIATION IN HUMAN BONE MARROW STEM CELLS
PALMIERI, Annalisa;CARINCI, Francesco;ZOLLINO, Ilaria;ZAMBONI, Paolo;
2012
Abstract
Synthetic and biological materials are increasingly used to provide temporary or permanent scaffolds for bone regeneration. Allogro, a demineralized freeze-dried bone allograft is an optimal scaffold used worldwide to fill bone defects and to restore bone loss in orthopedic and maxillofacial surgery. To study how Allogro can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction. The obtained results demonstrated that Allogro influences the osteo-differentiation of stem cells as demonstrated by the activation of osteoblast related genes RUNX2, ALPL and SPP1.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
