EFFECT OF ALGIPORE ON BONE MARROW STEM CELLS: AN IN VITRO STUDY

Cell-based bone and cartilage replacement is an evolving therapy aiming at the treatment of craniofacial and orthopedic defects (limb amputation, damaged tissues, bone and cartilage-related disorders and dental and maxillofacial reconstructive surgery). Autologous cell transplantation in combination with a biodegradable scaffold is a useful and safe option Algipore, a hydroxyapatite ceramic obtained from red alga, is largely employed as scaffolds in bone regeneration. Here we studied how this biomaterial promote osteoblast differentiation in stem cells derived from bone marrow, measuring the expression levels of bone related genes and mesenchymal stem cells marker by Real Time Reverse Transcription-Polymerase Chain Reaction. Algipore induces osteoblast differentiation in stem cells derived from bone marrow, as indicated by the activation of osteoblast related genes SPP1, ALPL, RUNX2 and SP7.