Pituitary tumors are mostly benign, being locally invasive in 5-35% of cases. Deregulation of several genes has been suggested as a possible alteration underlying the development and progression of pituitary tumors. Recently we have demonstrated that Magmas is over-expressed in a mouse ACTH-secreting pituitary adenoma cell line. Here we investigate by RT-QPCR the expression of Magmas in 4 rat pituitary adenoma cell lines: three growth hormone (GH) and prolactin (PRL)-secreting cells lines (GH1, GH3, and GH4C1) and one PRL-secreting cell line (MMQ). We found that Magmas is over-expressed in GH3 (2.2 fold) and MMQ (3.6 fold) cell lines, while GH1 and GH4C1 display Magmas expression levels similar to those detected in a pool of normal rat pituitaries. These results were confirmed by Western blot analysis that showed the same expression pattern found by RT-QPCR. Magmas mRNA expression was also assessed in 29 human pituitary tissues, including 19 nonfunctioning, 8 GH-secreting, 2 PRL-secreting, and one TSH-secreting pituitary adenoma, as well as in a pool of human normal pituitary mRNAs. We found that 24 out of 29 pituitary adenomas (82.7%) displayed a Magmas mRNA expression level >2-fold than that detected in a pool of human normal pituitaries (from 2 to 30-fold), in all types of pituitary adenomas, except for the TSH-secreting pituitary adenoma. Our results suggest that Magmas could be a novel tumor selective over-expressed gene and could be a novel molecular target to be studied in order to understand pituitary adenomas pathophysiolog
Magmas, a Novel Over-Expressed Gene in Pituitary Rat Cell Lines and in Human Pituitary Adenomas: A New Pituitary Biomarker?
TAGLIATI, Federico;GAGLIANO, Teresa;ROSSI, Rachele;DEGLI UBERTI, Ettore;ZATELLI, Maria Chiara
2011
Abstract
Pituitary tumors are mostly benign, being locally invasive in 5-35% of cases. Deregulation of several genes has been suggested as a possible alteration underlying the development and progression of pituitary tumors. Recently we have demonstrated that Magmas is over-expressed in a mouse ACTH-secreting pituitary adenoma cell line. Here we investigate by RT-QPCR the expression of Magmas in 4 rat pituitary adenoma cell lines: three growth hormone (GH) and prolactin (PRL)-secreting cells lines (GH1, GH3, and GH4C1) and one PRL-secreting cell line (MMQ). We found that Magmas is over-expressed in GH3 (2.2 fold) and MMQ (3.6 fold) cell lines, while GH1 and GH4C1 display Magmas expression levels similar to those detected in a pool of normal rat pituitaries. These results were confirmed by Western blot analysis that showed the same expression pattern found by RT-QPCR. Magmas mRNA expression was also assessed in 29 human pituitary tissues, including 19 nonfunctioning, 8 GH-secreting, 2 PRL-secreting, and one TSH-secreting pituitary adenoma, as well as in a pool of human normal pituitary mRNAs. We found that 24 out of 29 pituitary adenomas (82.7%) displayed a Magmas mRNA expression level >2-fold than that detected in a pool of human normal pituitaries (from 2 to 30-fold), in all types of pituitary adenomas, except for the TSH-secreting pituitary adenoma. Our results suggest that Magmas could be a novel tumor selective over-expressed gene and could be a novel molecular target to be studied in order to understand pituitary adenomas pathophysiologI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
