Autologous cell transplantation in combination with a biodegradable scaffold is one of the most promising bone regeneration techniques in craniofacial and orthopedic surgery. Algipore®, a hydroxyapatite ceramic obtained from red alga, is largely employed as scaffolds in bone regeneration. For this reason, we studied how Algipore® can induce osteoblast differentiation in stem cells derived from dental pulp, measuring the expression levels of bone related genes and mesenchymal stem cells marker by real time RT-PCR. The obtained results demonstrated that Algipore® enhance differentiation and deposition of matrixin stem cells by the activation of osteoblast related genes FOSL1 and SPP1 and the disappearance of the mesenchymal stem cells marker, ENG
DENTAL PULP DERIVED STEM CELLS DIFFERENTIATION AFTER ALGIPORE® TREATMENT
SOLLAZZO, Vincenzo;CARINCI, Francesco;PALMIERI, Annalisa;
2011
Abstract
Autologous cell transplantation in combination with a biodegradable scaffold is one of the most promising bone regeneration techniques in craniofacial and orthopedic surgery. Algipore®, a hydroxyapatite ceramic obtained from red alga, is largely employed as scaffolds in bone regeneration. For this reason, we studied how Algipore® can induce osteoblast differentiation in stem cells derived from dental pulp, measuring the expression levels of bone related genes and mesenchymal stem cells marker by real time RT-PCR. The obtained results demonstrated that Algipore® enhance differentiation and deposition of matrixin stem cells by the activation of osteoblast related genes FOSL1 and SPP1 and the disappearance of the mesenchymal stem cells marker, ENGI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
