Tissue engineering for bone grafting may emerge as an alternative to autogenous bone grafts. Allogro® a demineralized freeze-dried bone allograft is useful as a scaffold to restore bone loss in orthopedic and maxillofacial surgery. In order to get more inside how Allogro® can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in human osteoblasts and dental pulp stem cells, using real time RT-PC. The obtained results demonstrated that Allogro® enhance stem cells differentiation and deposition of matrix by the activation of osteoblast related genes FOSL1 and SPP1 and the disappearance of the mesenchymal stem cells marker, ENG
ALLOGRO® OSTEOGENIC POTENTIAL EVALUATION IN DERIVED PULP STEM CELLS
SOLLAZZO, Vincenzo;CARINCI, Francesco;PALMIERI, Annalisa;
2011
Abstract
Tissue engineering for bone grafting may emerge as an alternative to autogenous bone grafts. Allogro® a demineralized freeze-dried bone allograft is useful as a scaffold to restore bone loss in orthopedic and maxillofacial surgery. In order to get more inside how Allogro® can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in human osteoblasts and dental pulp stem cells, using real time RT-PC. The obtained results demonstrated that Allogro® enhance stem cells differentiation and deposition of matrix by the activation of osteoblast related genes FOSL1 and SPP1 and the disappearance of the mesenchymal stem cells marker, ENGI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
