Tricalcium Phosphate (TCP) is used successfully as bone substitutes and scaffolds for tissue engineering and implantology to its capacity to enhance bone formation in vivo. To study how TCP can induce osteoblast differentiation and proliferation in mesenchymal stem cells, the expression levels of bone related genes were mesasured in adipose derived stem cells (ADSCs) and normal osteoblast(NO) cultivated on TCP scaffolds after 15 and 30 days of treatment using real time Reverse Transcription-Polymerase Chain Reaction. Significantly, differentially expressed genes among ADSCs and NO were RUNX,COL1A1,ALPL and SPP1 in the first 15 days of tratment and SP7,FOSL1,RUNX2,COL3A1,COL1A1,SPP1 and ALPL after 30 days. The present study demonstrated that TCP influences the behavior ofADSCs in vitro by enhancing proliferation, differentiation and deposition of matrix
TRICALCIUM PHOSPHATE STIMULATES ADIPOSE TISSUE-DERIVED STEM CELLS TOWARDS OSTEOBLASTS DIFFERENTIATION
SOLLAZZO, Vincenzo;MELLONI, Elisabetta;ZAULI, Giorgio;CARINCI, Francesco
2011
Abstract
Tricalcium Phosphate (TCP) is used successfully as bone substitutes and scaffolds for tissue engineering and implantology to its capacity to enhance bone formation in vivo. To study how TCP can induce osteoblast differentiation and proliferation in mesenchymal stem cells, the expression levels of bone related genes were mesasured in adipose derived stem cells (ADSCs) and normal osteoblast(NO) cultivated on TCP scaffolds after 15 and 30 days of treatment using real time Reverse Transcription-Polymerase Chain Reaction. Significantly, differentially expressed genes among ADSCs and NO were RUNX,COL1A1,ALPL and SPP1 in the first 15 days of tratment and SP7,FOSL1,RUNX2,COL3A1,COL1A1,SPP1 and ALPL after 30 days. The present study demonstrated that TCP influences the behavior ofADSCs in vitro by enhancing proliferation, differentiation and deposition of matrixI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
