PerioGlas® (PG) is a silicate-based synthetic bone augmentation material used to fill periodontal defects, thanks its biocompatibility and osteoconductive properties. PG induces osteoblast proliferation and stem cells commitment thus it was hypotized that could be used as a template for the formation of bioengineered bone tissue. To study how PG can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in normal osteoblasts and dental pulp stem cells, using real time RT-PCR. Gene differentially expressed between the two cells type were the transcriptional factor SP7, osteopontin (SPP1), collagen type 1α1 (COL1A1), alkaline phosphatase (ALPL) and FOS-like antigen 1 (FOSL1). The present study demonstrated that PG influences the behavior of stem cells in vitro by enhancing proliferation and osteoblast differentiation
OSTEOBLAST DIFFERENTIATION OF DENTAL PULP STEM CELLS AFTER PERIOGLASS TREATMENT
CARINCI, Francesco;PALMIERI, Annalisa;SOLLAZZO, Vincenzo
2011
Abstract
PerioGlas® (PG) is a silicate-based synthetic bone augmentation material used to fill periodontal defects, thanks its biocompatibility and osteoconductive properties. PG induces osteoblast proliferation and stem cells commitment thus it was hypotized that could be used as a template for the formation of bioengineered bone tissue. To study how PG can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in normal osteoblasts and dental pulp stem cells, using real time RT-PCR. Gene differentially expressed between the two cells type were the transcriptional factor SP7, osteopontin (SPP1), collagen type 1α1 (COL1A1), alkaline phosphatase (ALPL) and FOS-like antigen 1 (FOSL1). The present study demonstrated that PG influences the behavior of stem cells in vitro by enhancing proliferation and osteoblast differentiationI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
