Effect of Engipore® treatment on adipose tissue-derived stem cells: an vitro study

Engipore® is a new bioactive material obtained using nanotechnologies, with architecture very similar to the natural bone. It is largely employed in bone regeneration in orthopedics and maxillofacial surgery because gradually resorbed by the osteoclast and replaced by new bone through osteoblastic activity. The molecular mechanism by which this material influences the behavior of osteoblast promoting proliferation and bone formation is poorly understood. To attempt this question we study the expression level of bone related genes in mesenchymal stem cells derived from adipose tissue and treated with Engipore® for 15 and 30 days. Gene expression profile, obtained wit real time RT-PCR, demonstrated that Engipore® influences the differentiation of adipose derived stem cells by the activation of osteoblast related genes SP7 and ALPL and the disappearance of the mesenchymal stem cells marker, ENG.