We report on the short tandem repeat analysis (STR), using PowerPlex ESX 16 Systems (Promega), of the supposed specimens belonged to M. B. died in 1980. DNA was extracted from three different specimens dating back to the 50s represented by two bloodstains found on two different fragments of fabric and a bloodstain on a cotton glove. As reference sample, we used a bone collected from the body. Before DNA extraction, in order to get rid modern DNA contaminations, the bone was treated with diluited bleach, water and UV light. The different STR profiles were confirmed using criteria currently available for validation of degraded DNA, including independent PCR replication. The results obtained after the genetic analysis confirmed that the specimens analized, rapresented by two bloodstains found on two different fragments of fabric and a bloodstain on a cotton glove, were directly referred to M. B..
Genetic analysis regarding the attribution of specimens covered ex Codice Iuris Canonic
FABBRI, Matteo;VENTURI, Marina;MARINELLI, Lorenzo;GAUDIO, Rosa Maria;AVATO, Francesco Maria
2011
Abstract
We report on the short tandem repeat analysis (STR), using PowerPlex ESX 16 Systems (Promega), of the supposed specimens belonged to M. B. died in 1980. DNA was extracted from three different specimens dating back to the 50s represented by two bloodstains found on two different fragments of fabric and a bloodstain on a cotton glove. As reference sample, we used a bone collected from the body. Before DNA extraction, in order to get rid modern DNA contaminations, the bone was treated with diluited bleach, water and UV light. The different STR profiles were confirmed using criteria currently available for validation of degraded DNA, including independent PCR replication. The results obtained after the genetic analysis confirmed that the specimens analized, rapresented by two bloodstains found on two different fragments of fabric and a bloodstain on a cotton glove, were directly referred to M. B..I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
