High and low grade cervical lesions were investigated for Polyomavirus (PYV) and Human Papillomavirus (HPV) sequences. In precancerous cervical lesions, the overall prevalence of PYV sequences was 44% (41/93). Specifically, among PYV-positive samples 83% (34/41) tested positive for BKV sequences, whereas 17% (7/41) were JCV-positive. None of the samples were SV40-positive. BKV DNA in HSIL was confirmed by in situ PCR experiments. BKV sequences were more frequently detected in HSIL, together with HPV-16. The association of BKV with precancerous cervical lesion suggests that this polyomavirus may participate, together with HPV-16, in the cell transformation process. Alternatively, BKV may multiply better in HPV-16 positive cells from precancerous cervical lesions than in HPV-16 negative cells.
High prevalence of BK polyomavirus sequences in HPV-16 positive precancerous cervical lesions.
MARTINI, Fernanda;TOGNON, Mauro
2011
Abstract
High and low grade cervical lesions were investigated for Polyomavirus (PYV) and Human Papillomavirus (HPV) sequences. In precancerous cervical lesions, the overall prevalence of PYV sequences was 44% (41/93). Specifically, among PYV-positive samples 83% (34/41) tested positive for BKV sequences, whereas 17% (7/41) were JCV-positive. None of the samples were SV40-positive. BKV DNA in HSIL was confirmed by in situ PCR experiments. BKV sequences were more frequently detected in HSIL, together with HPV-16. The association of BKV with precancerous cervical lesion suggests that this polyomavirus may participate, together with HPV-16, in the cell transformation process. Alternatively, BKV may multiply better in HPV-16 positive cells from precancerous cervical lesions than in HPV-16 negative cells.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
