The present study evaluated human primary osteoblasts and two different osteoblast - like cell lines behaviour when cultured in presence of different hydroxyapatite-based (HA) biomaterials (SINTlifeTM - FIN-CERAMICA S.p.a., Faenza, Italy; Bio-OssTM, Geistlich Biomaterials, Woulhusen, Switzerland; BiostiteTM - GABA Vebas, San Giuliano Milanese, MI, Italy), focusing attention on the effect of HA/BiostiteTM in terms of modulation of osteoblastic differentiation. Analysis were about adhesion, proliferation and mineralization activity. Runt-related transcription factor 2 (Runx2), Estrogen Receptor alpha (ERalfa) expression and alkaline phosphatase activity (ALP) were measured as osteoblastic differentiation markers. Determination of viable cells was done with MTT colorimetric assay. Scanning electron microscopy (SEM) analysis was performed on biomaterial-treated cells. All hydroxyapatite-based biomaterials didn't affect cells morphology and viability, whereas only presence of HA/BiostiteTM improved cells adhesion, growth and differentiation. Adhesion and spreading of the primary cells on HA/BiostiteTM were the same showed by two different osteoblast - like cell lines. These results have important implications for both tissue-engineered bone grafts and enhancement of HA implants performance, to develop new teeth's supporting structure therapies and replacement.
Effect of hydroxyapatite-based biomaterials on human osteoblast phenotype.
TROMBELLI, Leonardo;PENOLAZZI, Maria Letizia;TORREGGIANI, Elena;FARINA, Roberto;LAMBERTINI, Elisabetta;VECCHIATINI, Renata;PIVA, Maria Roberta
2010
Abstract
The present study evaluated human primary osteoblasts and two different osteoblast - like cell lines behaviour when cultured in presence of different hydroxyapatite-based (HA) biomaterials (SINTlifeTM - FIN-CERAMICA S.p.a., Faenza, Italy; Bio-OssTM, Geistlich Biomaterials, Woulhusen, Switzerland; BiostiteTM - GABA Vebas, San Giuliano Milanese, MI, Italy), focusing attention on the effect of HA/BiostiteTM in terms of modulation of osteoblastic differentiation. Analysis were about adhesion, proliferation and mineralization activity. Runt-related transcription factor 2 (Runx2), Estrogen Receptor alpha (ERalfa) expression and alkaline phosphatase activity (ALP) were measured as osteoblastic differentiation markers. Determination of viable cells was done with MTT colorimetric assay. Scanning electron microscopy (SEM) analysis was performed on biomaterial-treated cells. All hydroxyapatite-based biomaterials didn't affect cells morphology and viability, whereas only presence of HA/BiostiteTM improved cells adhesion, growth and differentiation. Adhesion and spreading of the primary cells on HA/BiostiteTM were the same showed by two different osteoblast - like cell lines. These results have important implications for both tissue-engineered bone grafts and enhancement of HA implants performance, to develop new teeth's supporting structure therapies and replacement.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
