Oocyte selection with the highest competency represent a major goal in IVF. Several studies have demonstrated that non classical HLA class I HLA-G molecule modulation creates a tolerogenic microenvironment at the feto-maternal interface and it seems to be implicated in embryo implantation. This study investigated if soluble HLA-G molecules production by the cumulus-oocyte complex (COC) could be a marker of oocyte maturation. sHLA-G molecule levels were analyzed using Bio-Rad’s Bio-Plex assay in 152 COC supernatants obtained from 42 women and maturated by an “in vitro maturation procedure”. The presence of sHLA-G molecules was confirmed by Western blot technique. The results demonstrate detectable amounts of sHLA-G molecule ranging from 300 to 800 pg/ml in 14/73 (19%) COCs that generated mature oocytes and the complete absence of detectable sHLA-G antigens in the supernatants of COCs that corresponded to immature oocytes. The detection of sHLA-G molecules in the COC culture supernatants corresponding to matured oocytes is proposed as a possible marker to identify the gametes with a higher functionality. This non-invasive marker could be used in addition to morphological approaches to reduce the number of fertilized oocytes and transferred embryos.

Production of sHLA-G molecules by “in vitro” matured cumulus-oocyte complex

RIZZO, Roberta;STIGNANI, Marina;BORGATTI, Monica;GAMBARI, Roberto;BARICORDI, Olavio
2009

Abstract

Oocyte selection with the highest competency represent a major goal in IVF. Several studies have demonstrated that non classical HLA class I HLA-G molecule modulation creates a tolerogenic microenvironment at the feto-maternal interface and it seems to be implicated in embryo implantation. This study investigated if soluble HLA-G molecules production by the cumulus-oocyte complex (COC) could be a marker of oocyte maturation. sHLA-G molecule levels were analyzed using Bio-Rad’s Bio-Plex assay in 152 COC supernatants obtained from 42 women and maturated by an “in vitro maturation procedure”. The presence of sHLA-G molecules was confirmed by Western blot technique. The results demonstrate detectable amounts of sHLA-G molecule ranging from 300 to 800 pg/ml in 14/73 (19%) COCs that generated mature oocytes and the complete absence of detectable sHLA-G antigens in the supernatants of COCs that corresponded to immature oocytes. The detection of sHLA-G molecules in the COC culture supernatants corresponding to matured oocytes is proposed as a possible marker to identify the gametes with a higher functionality. This non-invasive marker could be used in addition to morphological approaches to reduce the number of fertilized oocytes and transferred embryos.
2009
Rizzo, Roberta; Dal Canto, M. B.; Stignani, Marina; Fadini, R.; Fumagalli, D.; Mignini Renzini, M.; Borgatti, Monica; Gambari, Roberto; Baricordi, Olavio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1378314
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