In the orthopaedic perspective, tissue engineering is focused on the development of innovative materials, whose action consists in recruiting bone progenitor cells and in stimulating their proliferation. In this study, we investigated the effects of hydroxylapatite (HA) biomaterials, which differ in composition and form, on human mesenchymal stem cells (hMSCs). Doubling time variability among hMSC samples and their passages was investigated in “in vitro” cultures. Flow cytometric analysis was performed to characterize cell population towards specific mesenchymal stem cell surface markers. hMSCs viability, biocompatibility, adhesion and proliferation on different biomaterials were also investigated. Furthermore, hMSCs morphology was evaluated during their growth on biomaterials by scanning electron microscopy (SEM) analysis. hMSCs cytoskeleton was investigated by immunofluorescence assay to evaluate the effects of biomaterials on cell structure and organization.
High Porosity Bioceramic is a Favourable Environment for the Adhesion and Proliferation of Human Mesenchymal Stem Cells.
MANFRINI, Marco;DI BONA, Cristina;CANELLA, Alessandro;TOGNON, Mauro
2009
Abstract
In the orthopaedic perspective, tissue engineering is focused on the development of innovative materials, whose action consists in recruiting bone progenitor cells and in stimulating their proliferation. In this study, we investigated the effects of hydroxylapatite (HA) biomaterials, which differ in composition and form, on human mesenchymal stem cells (hMSCs). Doubling time variability among hMSC samples and their passages was investigated in “in vitro” cultures. Flow cytometric analysis was performed to characterize cell population towards specific mesenchymal stem cell surface markers. hMSCs viability, biocompatibility, adhesion and proliferation on different biomaterials were also investigated. Furthermore, hMSCs morphology was evaluated during their growth on biomaterials by scanning electron microscopy (SEM) analysis. hMSCs cytoskeleton was investigated by immunofluorescence assay to evaluate the effects of biomaterials on cell structure and organization.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
