The effects of acute exposure to the herbicide terbuthylazine (3.55, 5.01 and 7.08 mg l-1) on the cells of farmed European sea bass, Dicentrarchus labrax L., were investigated by means of light and electron microscopy. In gills of treated fish, the numbers of chloride cells (CCs) and rodlet cells (RCs) increased significantly within 24 h and 48 h respectively; the intestine showed the largest increase in RCs linked to treatment and exposure time. In kidney, 24 h exposure induced a significant increase in RCs and the number and global area of macrophage aggregates (MAs). Treated fish displayed cellular and/or ultrastructural alterations in all the organs examined. In the gills necrosis, lamellar and cellular oedema, epithelial lifting, telangectasia, and fusion of secondary lamellae were encountered. The liver presented myelin-like figures, cytoplasmic rarefaction and acute cell swelling of hepatocytes. In both organs, the severity of damage was dose-dependent. In RCs of gills, intestine and kidney of exposed sea bass high cytoplasmic vacuolization, myelin-like figures, cristolysis and varying degrees of rodlet degeneration were observed. Extensive rodlet expulsion occurred in the gut lumen. Exposure to terbuthylazine also affected the renal tubular epithelial cells, which exhibited ‘blebs’. Damage to the intestinal epithelial cells was also observed.
Effects of experimental terbuthylazine exposure on the cells of Dicentrarchus labrax (L.).
SAYYAF DEZFULI, Bahram;SIMONI, Edi;GIARI, Luisa;
2006
Abstract
The effects of acute exposure to the herbicide terbuthylazine (3.55, 5.01 and 7.08 mg l-1) on the cells of farmed European sea bass, Dicentrarchus labrax L., were investigated by means of light and electron microscopy. In gills of treated fish, the numbers of chloride cells (CCs) and rodlet cells (RCs) increased significantly within 24 h and 48 h respectively; the intestine showed the largest increase in RCs linked to treatment and exposure time. In kidney, 24 h exposure induced a significant increase in RCs and the number and global area of macrophage aggregates (MAs). Treated fish displayed cellular and/or ultrastructural alterations in all the organs examined. In the gills necrosis, lamellar and cellular oedema, epithelial lifting, telangectasia, and fusion of secondary lamellae were encountered. The liver presented myelin-like figures, cytoplasmic rarefaction and acute cell swelling of hepatocytes. In both organs, the severity of damage was dose-dependent. In RCs of gills, intestine and kidney of exposed sea bass high cytoplasmic vacuolization, myelin-like figures, cristolysis and varying degrees of rodlet degeneration were observed. Extensive rodlet expulsion occurred in the gut lumen. Exposure to terbuthylazine also affected the renal tubular epithelial cells, which exhibited ‘blebs’. Damage to the intestinal epithelial cells was also observed.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
