Tocopherols, fatty acids and sterol distribution in wild Ecuadorian Theobroma subincanum (Sterculiaceae) seeds

Ecuadorian Theobroma subincanum (Sterculiaceae) seed parts were analyzed to determine quali-quantitative tocopherol distribution. Fatty acids and sterols in the embryos, teguments and endosperm were also evaluated with an aim to better-characterize the plant matrix for its potential use as a source of phytochemicals, for the cocoa butter processing industry and/or as a new source of low cost natural products in the cosmetic, drug and alimentary/nutraceutical industries. HPLC for tocopherols and gas-chromatography (GC), GC-mass spectrometry (MS) analyses for fatty acids profile (FAP) and phytosterols were performed. Tocopherols were particularly abundant in the embryo, with quali-quantitative data similar to wheat germ oil whereas, in the teguments and endosperm, the concentrations of tocopherols were lower. The fatty acid profile and phytosterol characterization of the seed parts showed qualitative homogeneous data. In the endosperm, 80% of the entire FAPconsisted of oleic and stearic acid while, among sterols, cycloartenol was more abundant in endosperm than in embryos and teguments. Accordingly, T. subincanum seeds can be proposed as possible substitutes in the cocoa processing industry and as a potential source of vitamin E isomers.