A commercial reversed-phase (RP) C18 HPLC column has been dynamically coated with the chiral selector N<n-decyI-L-spinacine and then loaded with copper(ll) ions. Several racemic mixtures of underivatized amino acids and oligopeptides were resolved on the column by chiral ligand-exchange chromatography. The most important experimental conditions affecting column efficiency, retention, and selectivity (temperature and mobile phase flow rate and composition) were extensively investigated.
Chiral Ligand-Exchange Chromatography on a RP-HPLC Column Coated with a New Chiral Selector Derived from L-Spinacine
REMELLI, Maurizio;CONATO, Chiara
2002
Abstract
A commercial reversed-phase (RP) C18 HPLC column has been dynamically coated with the chiral selector NFile in questo prodotto:
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