The preparative-scale regio- and stereo-specific oxidation of hydroxy groups and reduction of keto functions at C(3) of several C24 bile acids catalyzed by a new 3a-hydroxysteroid dehydrogenase (3a-HDH) is reported. The crude enzyme isolated from the cells of Pseudomonas paucimobilis revelead the presence of a further enzymatic fraction containing a secondary alcohol dehydrogenase (SADH) that has been used to recycle the cofactor.
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Data di pubblicazione: | 1999 | |
Titolo: | Regiospecific oxidoreductions catalyzed by a new Pseudomonas paucimobilis hydroxysteroid dehydrogenase | |
Autori: | BIANCHINI E; CHINAGLIA N; DEAN M.; GIOVANNINI P. P.; MEDICI A; PEDRINI P; POLI S. | |
Rivista: | TETRAHEDRON | |
Parole Chiave: | Bile acids; 3a-hydroxysteroid dehydrogenase; oxidation; reduction | |
Abstract: | The preparative-scale regio- and stereo-specific oxidation of hydroxy groups and reduction of keto functions at C(3) of several C24 bile acids catalyzed by a new 3a-hydroxysteroid dehydrogenase (3a-HDH) is reported. The crude enzyme isolated from the cells of Pseudomonas paucimobilis revelead the presence of a further enzymatic fraction containing a secondary alcohol dehydrogenase (SADH) that has been used to recycle the cofactor. | |
Handle: | http://hdl.handle.net/11392/1206781 | |
Appare nelle tipologie: | 03.1 Articolo su rivista |
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