This paper describes the production and characterization of cationic microparticles based on pullulan and starch for the delivery of nucleic acids.The microparticles were prepared by chemically cross-linkinking of a polymer solution dispersed in organic phase, followed by amination with N, N-diethyl-2-chloroethyl amine hydrochloride or N-glycidyl-N, N-dimethyl-N-methylammonium chloride. The association of desoxyribonucleotide Defibrotide (DFT) with positively charged microparticles was determined. The association capacity and the affinity of microspheres for DFT were investigated as a function of type of polysaccharide, content and basicity of the aminogroups. It was found that the both types of carriers synthetized display a high affinity for Defibrotide due to the high porosity of polysaccharide microspheres. In addition, the in vitro release kinetics from microspheres were determined.
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http://hdl.handle.net/11392/1200243
Titolo: | Aminated polysaccharide microspheres as DNA delivery systems |
Autori: | |
Data di pubblicazione: | 2003 |
Rivista: | |
Abstract: | This paper describes the production and characterization of cationic microparticles based on pullulan and starch for the delivery of nucleic acids.The microparticles were prepared by chemically cross-linkinking of a polymer solution dispersed in organic phase, followed by amination with N, N-diethyl-2-chloroethyl amine hydrochloride or N-glycidyl-N, N-dimethyl-N-methylammonium chloride. The association of desoxyribonucleotide Defibrotide (DFT) with positively charged microparticles was determined. The association capacity and the affinity of microspheres for DFT were investigated as a function of type of polysaccharide, content and basicity of the aminogroups. It was found that the both types of carriers synthetized display a high affinity for Defibrotide due to the high porosity of polysaccharide microspheres. In addition, the in vitro release kinetics from microspheres were determined. |
Handle: | http://hdl.handle.net/11392/1200243 |
Appare nelle tipologie: | 03.1 Articolo su rivista |