Dot-blot assay and indirect immunofluorescence (IIF) were compared with regard to their ability to detect Toxoplasma gondii in frozen and unfrozen bronchoaveolar lavage fluid (BALF) or induced sputum samples collected from 19 AIDS patients with or without toxoplasmic encphalitis (TE). Dot-blot detected T. gondii antigen in frozen BALF and induced sputa obtained from 11 out of 14 patients (78.5 %) who had had TE, whereas IIF detected the parasite in frozen BALF samples only. In unfrozen samples, both techniques gave positive results with induced sputa collected from 3 of 5 patients (60%) without TE, all having clinical and laboratory findings of interstitial pneumonia. These specimens also were positive for toxoplasma either by Giemsa stain or by tissue culture isolation.
LABORATORY DIAGNOSIS OF PULMONARY TOXOPLASMOSIS BY DEMONSTRATION OF TOXOPLASMA-GONDII ANTIGEN IN LUNG SECRETIONS FROM PATIENTS WITH AIDS
CONTINI, Carlo;CULTRERA, Rosario;
1995
Abstract
Dot-blot assay and indirect immunofluorescence (IIF) were compared with regard to their ability to detect Toxoplasma gondii in frozen and unfrozen bronchoaveolar lavage fluid (BALF) or induced sputum samples collected from 19 AIDS patients with or without toxoplasmic encphalitis (TE). Dot-blot detected T. gondii antigen in frozen BALF and induced sputa obtained from 11 out of 14 patients (78.5 %) who had had TE, whereas IIF detected the parasite in frozen BALF samples only. In unfrozen samples, both techniques gave positive results with induced sputa collected from 3 of 5 patients (60%) without TE, all having clinical and laboratory findings of interstitial pneumonia. These specimens also were positive for toxoplasma either by Giemsa stain or by tissue culture isolation.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
