A reverse transcriptase-polymerase chain reaction (RT-PCR) assay, was performed to evaluate the transcription degree of bradyzoite-specific genes of 65 kDa Toxoplasma gondii protein. This method was compared with nested DNA amplification (n)-PCR. New designed oligonucleotide primers and probes, which identify a 212 bp fragment inside to the open reading MAG1 sequence, were employed in both RT-PCR and n-PCR assays. RT-PCR has been shown to be more sensitive and specific than n-PCR.
Expression of toxoplasmic 65 kDa cystic mRNA by RT-PCR in patients with Toxoplasma gondii infection relapses
CULTRERA, Rosario;SERACENI, Silva;SEGALA, Daniela;CONTINI, Carlo
2001
Abstract
A reverse transcriptase-polymerase chain reaction (RT-PCR) assay, was performed to evaluate the transcription degree of bradyzoite-specific genes of 65 kDa Toxoplasma gondii protein. This method was compared with nested DNA amplification (n)-PCR. New designed oligonucleotide primers and probes, which identify a 212 bp fragment inside to the open reading MAG1 sequence, were employed in both RT-PCR and n-PCR assays. RT-PCR has been shown to be more sensitive and specific than n-PCR.File in questo prodotto:
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