The suggested role of cAMP in the regulation of amnionic prostaglandin release was investigated using two adenylate cyclase inhibitors, MDL 12330A and SQ 22536. These substances exhibited a dose-dependent inhibitory effect on both amnionic enzyme and cAMP levels, but they did not influence prostaglandin E (PGE) release. In addition forskolin and IBMX (3-isobutyl-1-methylxanthine), two drugs known to increase cAMP levels, did not affect PGE output, while dibutyryl cyclic cAMP showed a dose-dependent inhibitory effect. On the basis of our data, the suggested role of amnionic adenylate cyclase in triggering prostaglandin release is not confirmed, and the pathway of phospholipase A 2 activation at the onset of labor remains to be elucidated.

THE CONTROVERSIAL ROLE OF CAMP ON AMNIOTIC PROSTAGLANDIN RELEASE - EFFECT OF ADENYLATE-CYCLASE INHIBITION

VESCE, Fortunato;BIONDI, Carla;FERRETTI, Maria Enrica;PARESCHI, Maria Cristina;BUZZI, Marco;
1995

Abstract

The suggested role of cAMP in the regulation of amnionic prostaglandin release was investigated using two adenylate cyclase inhibitors, MDL 12330A and SQ 22536. These substances exhibited a dose-dependent inhibitory effect on both amnionic enzyme and cAMP levels, but they did not influence prostaglandin E (PGE) release. In addition forskolin and IBMX (3-isobutyl-1-methylxanthine), two drugs known to increase cAMP levels, did not affect PGE output, while dibutyryl cyclic cAMP showed a dose-dependent inhibitory effect. On the basis of our data, the suggested role of amnionic adenylate cyclase in triggering prostaglandin release is not confirmed, and the pathway of phospholipase A 2 activation at the onset of labor remains to be elucidated.
Vesce, Fortunato; Biondi, Carla; Ferretti, Maria Enrica; Pareschi, Maria Cristina; Buzzi, Marco; Colamussi, Ml
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1197071
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