The fermentation of different sugars by Bacillus stearothermophilus ATCC 2027 to obtain 2,3-butanediol is described. Fermentation tests in shake flasks show the complete conversion of sucrose to BD (91% yield) and acetoin (3-hydroxy-2-butanone) using a starting sugar concentration of 30 g/L. A similar result is obtained with sugar cane molasses, whereas glucose and fructose afford lower yields. Other disaccharides and monosaccharides are also tested without appreciable results. B. stearothermophilus forms a mixture of 2R,3R-butanediol and the meso isomer derived both from reduction of R-AC and from the BD cycle as demonstrated by the presence of AC reductase and acetylacetoin synthase.

Production of 2,3-butanediol by Bacillus stearothermophilus: fermentation and metabolic pathway

GIOVANNINI, Pier Paolo;MANTOVANI, Matteo;PEDRINI, Paola;MEDICI, Alessandro
2008

Abstract

The fermentation of different sugars by Bacillus stearothermophilus ATCC 2027 to obtain 2,3-butanediol is described. Fermentation tests in shake flasks show the complete conversion of sucrose to BD (91% yield) and acetoin (3-hydroxy-2-butanone) using a starting sugar concentration of 30 g/L. A similar result is obtained with sugar cane molasses, whereas glucose and fructose afford lower yields. Other disaccharides and monosaccharides are also tested without appreciable results. B. stearothermophilus forms a mixture of 2R,3R-butanediol and the meso isomer derived both from reduction of R-AC and from the BD cycle as demonstrated by the presence of AC reductase and acetylacetoin synthase.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/527586
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