Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replication, the protein was produced. The purified U94/REP retained the characteristic immunological features. It was internalized and localized in the nucleus of human cells, showing marked inhibitory activity on the replication of HHV-6 (both variants A and B). The effect of U94/REP was dose-dependent and sensitive to treatment with single-stranded but not double-stranded DNA. U94/REP inhibited the replication of other betaherpesviruses, HHV-7 and human cytomegalovirus, but had no effect on herpes simplex virus. These results confirm the action of U94/rep latency gene in the regulation of HHV-6 replication with implications for co-reactivations and latency of human betaherpesviruses.

Human herpesvirus 6 (HHV-6) U94/REP protein inhibits betaherpesvirus replication

CASELLI, Elisabetta
Primo
;
GALVAN, MONICA;BONI, Michela;ROTOLA, Antonella;BERGAMINI, Carlo;CASSAI, Enzo
Penultimo
;
DI LUCA, Dario
Ultimo
2006

Abstract

Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replication, the protein was produced. The purified U94/REP retained the characteristic immunological features. It was internalized and localized in the nucleus of human cells, showing marked inhibitory activity on the replication of HHV-6 (both variants A and B). The effect of U94/REP was dose-dependent and sensitive to treatment with single-stranded but not double-stranded DNA. U94/REP inhibited the replication of other betaherpesviruses, HHV-7 and human cytomegalovirus, but had no effect on herpes simplex virus. These results confirm the action of U94/rep latency gene in the regulation of HHV-6 replication with implications for co-reactivations and latency of human betaherpesviruses.
2006
Caselli, Elisabetta; Bracci, A; Galvan, Monica; Boni, Michela; Rotola, Antonella; Bergamini, Carlo; Cermelli, C; DAL MONTE, P; Gompels, Ua; Cassai, Enzo; DI LUCA, Dario
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/499052
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