Mass-transfer kinetics in a shell packing material for chromatography

A shell particle consists of a solid, nonporous core that is surrounded with a shell of a porous solid having essentially the same physicochemical properties as those of the conventional porous particles used as packing media in chromatography. The diameter of the solid core and the thickness of its shell or the external diameter of the particle characterizes the chromatographic properties of the packing material. The potential advantage of this particle structure would be the shorter average path length experienced by solute molecules during their diffusion across the particles of packing material when they are retained. Compounds having slow pore diffusion would exhibit higher efficiencies on columns packed with shell than with conventional, fully porous particles. Using columns packed with Halo, a new type of porous silica shell particles, we assess the gain achieved with this principle for peptides of moderate molecular weights and for small proteins.