An invasive cervical carcinoma was found to harbor an episomal variant of human papillomavirus (HPV) type 16 DNA, with a size of about 10.1 kb. A genomic library of the tumor was constructed in bacteriophage lambda and a recombinant phage clone was isolated by screening with HPV 16 probe. Analysis by restriction mapping and Southern hybridization showed that the isolate contained a 2.2 kb duplication of the early region, which included part of E6, all E7 and part of E1 open reading frames. Possible consequences of this duplication for oncogenesis are discussed. © 1989.

Episomal HPV 16 DNA isolated from a cervical carcinoma presents a partial duplication of the early region

DI LUCA, Dario
Primo
;
CASELLI, Elisabetta
Secondo
;
ROTOLA, Antonella;CASSAI, Enzo
Ultimo
1989

Abstract

An invasive cervical carcinoma was found to harbor an episomal variant of human papillomavirus (HPV) type 16 DNA, with a size of about 10.1 kb. A genomic library of the tumor was constructed in bacteriophage lambda and a recombinant phage clone was isolated by screening with HPV 16 probe. Analysis by restriction mapping and Southern hybridization showed that the isolate contained a 2.2 kb duplication of the early region, which included part of E6, all E7 and part of E1 open reading frames. Possible consequences of this duplication for oncogenesis are discussed. © 1989.
1989
DI LUCA, Dario; Caselli, Elisabetta; Monini, P; Rotola, Antonella; Savioli, A; Cassai, Enzo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/470295
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