In this study, a microscopic probabilistic model of chromatography that establishes a conceptual link between single-molecule dynamics observation at liquid–solid interfaces and chromatographic experiments is described. This model is based on the discrete Lévy representation of stochastic processes and has the great advantage that it can be directly applied to the raw data set of single-molecule observations. The information contained in the molecular measurements includes some erratic rare events that are potentially very informative. Because experimental data need not be processed by mathematical–statistical transformation, application of this model preserves all the information that could be lost in an ensemble-averaged representation. In this approach, single-molecule experiments and stochastic interpretation are combined. It is of great importance to investigate superficial and interfacial phenomena in different areas, such as adsorption mechanisms in chromatography and mechanisms of biological activity, and to track the behavior of individual molecules in living cells.

Microscopic models of liquid chromatography: From ensemble-averaged information to resolution of fundamental viewpoint at single-molecule level

PASTI, Luisa
Primo
;
MARCHETTI, Nicola
Secondo
;
GUZZINATI, Roberta;CATANI, Martina;BOSI, Valentina;CAVAZZINI, Alberto
Ultimo
2016

Abstract

In this study, a microscopic probabilistic model of chromatography that establishes a conceptual link between single-molecule dynamics observation at liquid–solid interfaces and chromatographic experiments is described. This model is based on the discrete Lévy representation of stochastic processes and has the great advantage that it can be directly applied to the raw data set of single-molecule observations. The information contained in the molecular measurements includes some erratic rare events that are potentially very informative. Because experimental data need not be processed by mathematical–statistical transformation, application of this model preserves all the information that could be lost in an ensemble-averaged representation. In this approach, single-molecule experiments and stochastic interpretation are combined. It is of great importance to investigate superficial and interfacial phenomena in different areas, such as adsorption mechanisms in chromatography and mechanisms of biological activity, and to track the behavior of individual molecules in living cells.
2016
Pasti, Luisa; Marchetti, Nicola; Guzzinati, Roberta; Catani, Martina; Bosi, Valentina; Dondi, F; Sepsey, A; Felinger, A; Cavazzini, Alberto
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S016599361500268X-main.pdf

solo gestori archivio

Descrizione: Full text ed
Tipologia: Full text (versione editoriale)
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 814.41 kB
Formato Adobe PDF
814.41 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
11392-2351265_POSTPRINT_PASTI_LUISA.pdf

accesso aperto

Descrizione: Post print
Tipologia: Post-print
Licenza: Creative commons
Dimensione 961.85 kB
Formato Adobe PDF
961.85 kB Adobe PDF Visualizza/Apri

I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2351265
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 12
social impact