Lectins, proteins that bind the specific carbohydrate structures, are ubiquitous and are found in animals, plants and microorganisms. Eight biotinylated lectins linking carbohydrates were used on the deparaffinised, hydrated uninfected and helminth-infected intestinal sections of chub Squalius cephalus and brown trout, Salmo trutta. The reaction of each lectin was determined through the use of avidin DH-biotinylated horseradish peroxidase and the DAB chromogen. A diverse array of lectin/carbohydrate responses were seen across the two fish species. DBA, PNA, UEA I and WGA were seen on trout mucous cells, while only DBA and PNA were seen on the mucous cells in chub. Parasite presence was seen to induce changes in the mucus carbohydrate composition of the fish's intestine.Numerous mucous cells reactive to DBA-PNA and UEA I subsequently appeared in parasitised chub and trout. The responsiveness of the mucous cells, however, is not dependent on the type of parasite infecting the host, as similar lectin patterns were seen in trout intestines infected with acanthocephalans and cestodes. Lectin histochemistry also highlights the responsiveness of mast cells (MCs), a type of granulocyte which are present in most tissues and are strategically positioned near blood vessels, in the connective tissue of chub (PNA-SNA) and trout (SNA). This comparative study between cestodes and acanthocephalans and their effects on the distribution of various lectins in the intestines of two fish represents a first record.

Lectin histochemistry of intestinal mucous and mast cells in helminth-infected fish.

SAYYAF DEZFULI, Bahram;GIARI, Luisa;
2015

Abstract

Lectins, proteins that bind the specific carbohydrate structures, are ubiquitous and are found in animals, plants and microorganisms. Eight biotinylated lectins linking carbohydrates were used on the deparaffinised, hydrated uninfected and helminth-infected intestinal sections of chub Squalius cephalus and brown trout, Salmo trutta. The reaction of each lectin was determined through the use of avidin DH-biotinylated horseradish peroxidase and the DAB chromogen. A diverse array of lectin/carbohydrate responses were seen across the two fish species. DBA, PNA, UEA I and WGA were seen on trout mucous cells, while only DBA and PNA were seen on the mucous cells in chub. Parasite presence was seen to induce changes in the mucus carbohydrate composition of the fish's intestine.Numerous mucous cells reactive to DBA-PNA and UEA I subsequently appeared in parasitised chub and trout. The responsiveness of the mucous cells, however, is not dependent on the type of parasite infecting the host, as similar lectin patterns were seen in trout intestines infected with acanthocephalans and cestodes. Lectin histochemistry also highlights the responsiveness of mast cells (MCs), a type of granulocyte which are present in most tissues and are strategically positioned near blood vessels, in the connective tissue of chub (PNA-SNA) and trout (SNA). This comparative study between cestodes and acanthocephalans and their effects on the distribution of various lectins in the intestines of two fish represents a first record.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2330464
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